Homogeneously staining regions in direct preparations from human neuroblastomas.

Homogeneously Staining Regions in Direct Preparations from Human Neuroblastomas1

Previous descriptions of homogeneously staining regions in human neuroblastomas have been in karyotypes obtained from established cell lines. We now report homogeneously staining regions in direct preparations from two human neuroblastomas. In one of the cases, the homogeneously staining region iden tified in the primary tumor was also found in métastases to bone marrow and pleural fluid. The ...

Human neuroblastomas and abnormalities of chromosomes 1 and 17.

Structural rearrangements of chromosome 1p have been reported previously as a frequent finding in human neuroblastomas. In a review of karyotypes from 35 neuroblastomas (including 29 published cases and 6 unpublished tumors and cell lines), it was found that, in addition to the abnormalities of chromosome 1p (found in approximately 70% of cases), abnormalities involving only 2 other chromosome ...

Duplication of N-MYC at its resident site 2p24 may be a mechanism of activation alternative to amplification in human neuroblastoma cells.

Amplification of the human N-MYC proto-oncogene is frequently seen either in extrachromosomal double minutes or in homogeneously staining regions of aggressively growing neuroblastomas. N-MYC maps to chromosome 2 band p23-24, but homogeneously staining regions have never been observed at this band, suggesting transposition of N-MYC during amplification. Previous studies had suggested that in ce...

Homogeneously Staining Regions or Double Minute Chromosomes in Leukemia (Brief Communication)

MYCN is retained in single copy at chromosome 2 band p23-24 during amplification in human neuroblastoma cells.

Amplification of the human N-myc protooncogene, MYCN, is frequently seen either in extrachromosomal double minutes or in homogeneously staining regions of aggressively growing neuroblastomas. MYCN maps to chromosome 2 band p23-24, but homogeneously staining regions have never been observed at this band, suggesting transposition of MYCN during amplification. We have employed fluorescence in situ...